You would have to do it in after effects (preferred) or directory (though I am not positive about it. Won't work in flash - you can't get high enough quality for broadcast video.
Unless they are LCD projectors running off of a computer, in which case do it in flash and bump up size and resolution of your files in photoshop (150dpi).
For those sizes tweening the animation in frames might not work to well, you might have to program the bounce effects and such in AS (if you are not already).
Cheers.